INTRACELLULAR LOCALIZATION OF LIPOPROTEIN LIPASE ACTIVITY IN RAT HEART

Resumen

The intracellular localization of a lipoprotein lipase (LPL) activity in rat heart was shown. A crude separation showed the maximal total activity in the nuclear fraction and the maximal specific activity in the microsomal fraction. Each subcellular fraction was purified further and LPL activity remained only demonstrable in the
microsomal fraction. A further fractionation of the microsomal fraction was attempted, obtaining the membranous  fraction of the sarcoplasmic reticulum in a pure form. This was shown to be the site of the intracellular localization of LPL activity.